Diversity analysis by RAPD among the selected sunflower (Helianthus annus L.) parental lines
Author(s): Dhutmal RR, More AW, Kalpande HV and Maloo SR
Abstract: PCR amplifications from the 10 RAPD primers gave an average of 58.7 selected markers /primer. Amplification products were obtained in the range of 38-98. For OPD 05 primer, the highest number of fragments (98 amplicons) was detected, while OPW 15 primer revealed the least number (38 amplicons). A total of 587 amplicons were generated by the tested primers with an average of 58.7 amplicons/primer. Based on RAPD similarity matrix data (Table 2), the value of similarity coefficient ranged from 0.44 to 0.97 i.e., 44-97% or the genetic diversity ranged from 3-56%. The average similarity across all the genotypes was 0.73 showing that the genotypes were moderately similar genetically. Maximum similarity value of 0.97 was observed between genotypes 234B and 2-7-1B whereas, EC 623023 & EC 601957 were found to be genetically diverse with minimum similarity value of 0.44. Genotype EC 623023 showed low similarity values with most of the genotypes and was thus most diverse from rest of the genotypes. The RAPD cluster tree analysis of 14 Helianthus annus genotypes showed that they were mainly divided into two major clusters. The genotypes EC 623023, R 16, EC 601957 (testers) and CMS 234B and ARM 249B (lines) were spotted as genetically diverse since they are falling in different clusters based over RAPD marker analysis. Therefore could be efficiently utilized in improvement programmes.
Dhutmal RR, More AW, Kalpande HV, Maloo SR. Diversity analysis by RAPD among the selected sunflower (Helianthus annus L.) parental lines. Int J Stat Appl Math 2023;8(6S):1487-1491. DOI: 10.22271/maths.2023.v8.i6Ss.1754
